Work plan is divided into 9 WPs, including 4 technical WPs (WP2-5), and those dedicated to integration (WP6), validation (WP7), exploitation and dissemination of results (WP8), and project management (WP9).
WP1 will establish format, requirements, and performance criteria of extraction and detection modules
WP2 will develop efficient and specific magnetic extraction of exosomes from biological samples. And will establish modular microfluidic devices for system integration
WP3 will develop novel optical techniques for contrast enhancement of exosome scattering on layered substrates and will develop an optical sensor for discrete identification of individual nanoparticles down to 30 nm size.
WP4 will develop automated image processing algorithms to count and determine size of individual exosomes and will demonstrate exosome detection, size characterization, and phenotyping
WP5 will work on antibody immobilization strategies for immune-capture and non-destructive release of exosomes and will establish protocols for on-chip exosome analysis by antibody microarrays
WP6 will develop the software to automate fluidic steps and will Integrate the mechanical, fluidic and electrical parts of system components
WP7 will develop protocols for preparation of samples and will demonstrate the system on plasma from cohorts of healthy donors and lung cancer patients.
WP8 will plan a strategy to disseminate project findings to the scientific community and the general public and will set up a coordinated plan for exploitation/commercialization of deliverables obtained
WP9 will manage a structure for efficient operational management of administrative, financial and legal issues